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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
12/11/2020 |
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Data da última atualização: |
12/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HUL, L. M.; IBELLI, A. M. G.; PEIXOTO, J. de O.; SOUZA, M. R.; SAVOLDI, I. R.; MARCELINO D. E. P.; TREMEA, M.; LEDUR, M. C. |
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Afiliação: |
LUDMILA MUDRI HUL, UNICENTRO; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; MAYLA REGINA SOUZA, UDESC/Chapecó; IGOR RICARDO SAVOLDI, UDESC/Chapecó; DÉBORA ESTER PETRY MARCELINO, FACC; MATEUS TREMEA, UFSM; MONICA CORREA LEDUR, CNPSA. |
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Título: |
Reference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plos One, v. 15, n.8, Ed. e0238189, 2020. |
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DOI: |
https://doi.org/10.1371/journal.pone.0238189 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The use of reference genes is required for relative quantification in gene expression analysis and the stability of these genes can be variable depending on the experimental design. Therefore, it is indispensable to test the reliability of endogenous genes previously to their use. This study evaluated nine candidate reference genes to select the most stable genes to be used as reference in gene expression studies with the femoral cartilage of normal and epiphysiolysis-affected broilers. The femur articular cartilage of 29 male broilers with 35 days of age was collected, frozen and further submitted to RNA extraction and quantitative PCR (qPCR) analysis. The candidate reference genes evaluated were GAPDH, HMBS, HPRT1, MRPS27, MRPS30, RPL30, RPL4, RPL5, and RPLP1. For the gene stability evaluation, three software were used: GeNorm, BestKeeper and NormFinder, and a global ranking was generated using the function RankAggreg. In this study, the RPLP1 and RPL5 were the most reliable endogenous genes being recommended for expression studies with femur cartilage in broilers with epiphysiolysis and possible other femur anomalies. |
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Palavras-Chave: |
Epifisiólise femoral. |
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Thesagro: |
Cartilagem; Fêmur; Frango de Corte; Genética Animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01963naa a2200277 a 4500
001 2126548
005 2020-11-12
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0238189$2DOI
100 1 $aHUL, L. M.
245 $aReference genes for proximal femoral epiphysiolysis expression studies in broilers cartilage.$h[electronic resource]
260 $c2020
520 $aAbstract: The use of reference genes is required for relative quantification in gene expression analysis and the stability of these genes can be variable depending on the experimental design. Therefore, it is indispensable to test the reliability of endogenous genes previously to their use. This study evaluated nine candidate reference genes to select the most stable genes to be used as reference in gene expression studies with the femoral cartilage of normal and epiphysiolysis-affected broilers. The femur articular cartilage of 29 male broilers with 35 days of age was collected, frozen and further submitted to RNA extraction and quantitative PCR (qPCR) analysis. The candidate reference genes evaluated were GAPDH, HMBS, HPRT1, MRPS27, MRPS30, RPL30, RPL4, RPL5, and RPLP1. For the gene stability evaluation, three software were used: GeNorm, BestKeeper and NormFinder, and a global ranking was generated using the function RankAggreg. In this study, the RPLP1 and RPL5 were the most reliable endogenous genes being recommended for expression studies with femur cartilage in broilers with epiphysiolysis and possible other femur anomalies.
650 $aCartilagem
650 $aFêmur
650 $aFrango de Corte
650 $aGenética Animal
653 $aEpifisiólise femoral
700 1 $aIBELLI, A. M. G.
700 1 $aPEIXOTO, J. de O.
700 1 $aSOUZA, M. R.
700 1 $aSAVOLDI, I. R.
700 1 $aMARCELINO D. E. P.
700 1 $aTREMEA, M.
700 1 $aLEDUR, M. C.
773 $tPlos One$gv. 15, n.8, Ed. e0238189, 2020.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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